Collecting Hydrocarbons

Before cuticular hydrocarbons can be analysed they need to be removed from the cuticle of the insect. Extracting the hydrocarbons can be done in two different ways; liquid extracting or solid phase micro extraction (SPME). Extraction needs to be done carefully as if the method is too aggressive, many of the internal compounds from the insect will be extracted as well. Insects that are collected from plants or within their nests usually give clean extracts, but if material (larvae, eggs, maggots, larvae) are collected from corpses, this potentially could lead to some more dirty extracts as some of the corpse's tissue may have accumulated on the material. Millar and Sims (1998) give a very good review on the preparation, cleanup and fractionation of extractions.

0 0

Post a comment