Research and Development Component

The Biofabrica Moscamed Brasil is the first facility in Brazil to produce sterile insects on a large scale and, as a result, there is a small critical mass of trained personnel in the country. There is however, no information regarding the effectiveness of the SIT under semiarid conditions. A set of experiments and large-scale field tests were planned and are being carried out to answer basic questions concerning the mating competitiveness of tsl strain VIENNA 8 (Franz 2005), sterile males competing with wild males for wild females, dispersion and longevity of sterile males under semi-arid conditions and related topics. In collaboration with the Agricultural Research Service (ARS) of USDA, the University of Tessaly in Greece, and the Joint FAO/IAEA Programme, one staff member is leading a group in EMBRAPA to answer these questions with financial support from the Bank of Nordeste.

Large pilot tests are also planned for two

Table 1. Production and basic biological data of the VIENNA 8 genetic sexing strain colony at USP-CENA, Piracicaba, SP, Brazil.

Generation'

Eggs2

Egg

Number of

Male

Female

Sex error in

Sex error in

(month/year)

(ml)

viability

pupae

viability

viability

brown pupae

white pupae

(%)

(x 1000)

(%)

(%)

(%)

(%)

F10 (Dec/04)

12.5

24.7

48

63.1

85.1

1.18

0.00

F11(Jan/05)

19.1

77.2

1257

84.9

80.3

0.30

0.00

F12 (Feb/05)

64.3

75.8

1680

76.9

86.6

0.85

0.18

F13 (Mar/05)

52.2

63.8

1430

83.5

90.7

0.50

0.00

F14 (Apr/05)

98.2

71.6

1390

83.1

89.6

0.26

0.08

F15 (May/05)

243.5

68.3

3240

n.a.

n.a

n.a

n.a

'The VIENNA 8 pupae received from the FAO/ IAEA Agriculture and Biotechnology Laboratory were from generation F9 (Carlos Câceres, personal communication) ^Not all collected eggs were used for pupal production

'The VIENNA 8 pupae received from the FAO/ IAEA Agriculture and Biotechnology Laboratory were from generation F9 (Carlos Câceres, personal communication) ^Not all collected eggs were used for pupal production mango areas - 3000 and 5000 hectares -where the Mediterranean fruit fly is historically reported as being of economic importance. Sterile males will be brought from ISCAMEN and released in the experimental areas after a detailed survey and following suppression measures taken when the feral population is high. For these pilot tests, partnerships were established with EMBRAPA, the Bahia Animal and Plant Protection Agency (ADAB), and the University of south-western Bahia (Universidade Estadual do Sudoeste da Bahia (UESB)) to have good entomological and logistical support to carry them out.

At the USP-CENA laboratories, the VIENNA 8 strain received from the FAO/IAEA Agriculture and Biotechnology Laboratory in December 2004 was successfully adapted to a larval diet, which was developed in 2002 with local ingredients (Walder 2002). This diet contains sugarcane bagasse as a bulking agent, wheat germ and brewer yeast as protein sources, sugar and wheat flour as phagostimulants and carbohydrate sources, an antibiotic as bacterial growth inhibitor, sodium benzoate to avoid fungal growth and hydrochloric acid as pH regulator. The rearing protocol is very similar to that recommended by Câceres (2002). Studies using several quality tests including gamma sterilization are being applied under laboratory conditions (FAO/IAEA/USDA 2003). After six generations the strain shows good quality and stability (Table 1), enabling colony expansions to be planned for subsequent generations.

USP-CENA will be responsible for maintaining the original strain imported from the FAO/IAEA Agriculture and Biotechnology Laboratory and transferring it to the Biofâbrica Moscamed Brasil when the mass-rearing process starts. Also the tests for improving the local diet and the quality control of the rearing will be supervised by USP-CENA.

0 0

Post a comment