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Color Figure 15.3 The gut of a female Anopheles stephensi mosquito, colonized by transformed bacteria of the genus Asaia that express the green fluorescent protein (GFP). A massive colonization is located at the midgut level.

Color Figure 16.1 Details of stylets of phloem sucking insects and grapevine leaf tissues during biting. (A-D) Peryceria purchasi stylet penetrating grapevine tissues. (A-C) The pictures show the plasticity of the stylet that is inserted between cells in the parenchimatic tissue to reach the phloem cells. (D) Details of the stylet track (arrow) within the leaf tissue showing that the stylet of P. purchasi has explored different phloem tubes. (E-G) Biting of grapevine tissues by S. titanus. (E) The stylet of S. titanus penetrating the leaf tissues. (F and G) Tracks (arrows) left by the stylet of S. titanus in the vein of grapevine leaves

Color Figure 17.1 Healthy termite gut containing a dense protozoa population.

Color Figure 17.2a Deterioration of protozoa in the hindgut after workers were fed D-Hecate. 1 = vesicles inside affected protozoan. 2 = dead protozoan.

Color Figure 17.2b Defaunated hindgut.
Color Figure 17.3 Defaunation of worker hindgut after injection of lytic peptides.

Color Figure 18.2 Symbionts in insect cell culture and pure culture. Insect cell lines are useful for the culture of facultative symbionts and the study of interactions between symbionts and host cells. In plate A, Ca. Arsenophonus arthropodicus is attached to the surface of an Aedes albopictus C6/36 cell. The insect cell and bacteria were fixed and stained with FM4-64 (which binds to cellular lipids) and DAPI (which binds to nucleic acids) and visualized by deconvolution fluorescence microscopy. In plate B, live bacterial cells from a pure culture of S. glossinidius were visualized by fluorescence microscopy following staining with FM4-64 and DAPI. Pure culture isolation provides opportunities for the genetic manipulation of facultative symbionts.

Biological Sciences

CONTEMPORARY TOPICS in ENTOMOLOGY SERIES

THOMAS A. MILLER Editor

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